Total Coliform Sampling Guide

All water samples must be taken in approved, sterile sampling containers, which our laboratory can provide upon request. Treated (municipality/distribution systems) water samples must be de-chlorinated with sodium thio-sulfate (this is already in bottles that are provided by our laboratory). Keep sampling bottle closed until it is to be filled at the sampling location.

Distribution or sink tap sample

1. Remove aerator from faucet.
2. Rinse faucet with a bleach solution.
3. Flush line- by turning tap fully on and letting it run for 2 to 3 minutes, or for a time sufficient to permit clearing the service line.
4. Reduce water flow to permit filling bottle without splashing.
5. 100mL is needed for each sample submitted for analysis.

Well samples
If a sample is taken from a well fitted with a hand pump, pump water to waste for about 5 minutes before collecting sample. If the well is equipped with a mechanical pump, collect sample from a tap on the discharge. If there is no pumping machinery, collect sample directly from the well by means of a sterilized bottle fitted with a weight at the base; take care to avoid contaminating sample by any surface scum.