Guidance for Interpretation of Mold Culture Results

There are no government-issued numerical standards for mold culture interpretation. However, some environmental companies, industrial hygienists, and other IAQ professionals use the following arbitrary numbers for guidance in interpreting microbial survey results.

The final mold interpretation should not be based solely on numbers! Information gathered from the walk-through investigation of the area is very significant, including sources of moisture or high humidity, and signs of visible mold growth. In air samples, it is important to consider the type and concentration of fungi indoors, as compared to outdoors or a non-complaint area. One should consider the indoor: outdoor fungal count ratio, the presence/absence of certain fungi indoors versus outdoors, the genus/species of predominant fungi indoors versus outdoors, and whether the fungi detected indoors are allergenic and/or toxigenic.

Bioaerosol
<250CFU/m3Low/Normal
250-1,000CFU/m3Moderate/Borderline
>1,000CFU/m3Active Growth/Sporulation
>5,000

CFU/m3Very Active Growth/Sporulation
Swab/Wipe
<100CFU/in2No Growth/Background
<10,000CFU/in2Low/Normal Growth
10,000-100,000CFU/in2Moderate Growth
100,000-1,000,000CFU/in2Active Growth/Sporulation
>1,000,000

CFU/in2Very Active Growth/Sporulation
Bulk substrate
<100CFU/gNo Growth
<25,000CFU/gLow/Normal Growth
25,000-200,000CFU/gModerate Growth
200,000-1,000,000CFU/gActive Growth/Sporulation
>1,000,000

CFU/gVery Active Growth/Sporulation
Micro-vac Dust
<5,000CFU/ft2Low/Normal
5,000-25,000CFU/ft2Moderate/Borderline
25,000-75,000CFU/ft2Active Growth/Sporulation
>75,000

CFU/ft2Very Active Growth/Sporulation
Definitions
CFU = Colony Forming Unit
Colony = A mass of branching hyphae with or without spores, generally of one species and potentially started from one spore, cell, or propagule.

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